Lambda ISH
- Tech Only CPT
- Tech Pro CPT
- PowerPath Code SOO
- Schedule Monday - Friday
- Turn Around Time Within 2-5 business days of receipt
- Disease State Myeloma
- Methodology ISH
Specimen Requirements
Formalin-fixed, paraffin embedded tissue block or cell block, or 1 unstained charged slide for each test requested plus 3-4 additional unstained slides cut at 4 microns. Adequate space on each slide for control tissue is required.
This is a send out test.
Diagnostic Utility
The chromogenic in situ hybridization (CISH) uses a probe with sequence complimentary to a segment of lambda light chain mRNA. It is useful to identify lambda light chain mRNA in the cytoplasm of plasma cells and plasmacytoid lymphocytes, which in turn may help determining kappa or lambda light chain restriction, a surrogate for clonality. CISH has the advantage of avoiding background staining from serum frequently seen with antibody in immunohistochemistry.